Biochemical Studies on 5'-Nucleotidase of Schwann Cells in Degenerated Nerve

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Abstract
The partial characterization of 5''-nucleotidase (5''-AMPase) in Schwann-cell plasmalemmae (PM) prepared from degenerated cat sciatic nerve is described. 5''-AMPase was enriched 3.7-fold in the PM fraction over that of the crude homogenate preparation. The plant lectin concanavalin-A (Con-A) reduced Schwann cell PM 5''-AMPase activity in a concentration-dependent manner (30-600 .mu.g/ml). Plasma membrane 5''-AMPase activity was maximally inhibited to 20% of control values by Con-A (400-600 .mu.g/ml) and activity returned to control levels by pretreatment with the hapten sugar .alpha.-methyl-D-mannoside (50 mM). Equimolar concentrations of UDP and ADP (100 .mu.M) reduced the rate of hydrolysis of labeled AMPto labeled adenosine in PM to 45% and 35% of controls, respectively. This is the 1st study to characterize a Schwann-cell PM enzyme and demonstrates that 5''-AMPase may be use as a Schwann-cell PM enzyme and demonstrates that 5''-AMPase may be caused as a Schwann-cell PM marker enzyme.