Determination of the Antineoplastic Agent Tiazofurin in Plasma by High Performance Liquid Chromatography
- 31 December 1983
- journal article
- research article
- Published by Taylor & Francis in Analytical Letters
- Vol. 17 (7) , 607-615
- https://doi.org/10.1080/00032718408066494
Abstract
A simple and sensitive sample clean up procedure and a reverse phase high performance liquid chromatographic assay for tiazofurin in dog plasma has been developed. Thymidine was used as the internal standard and the elution solvent was 0.16 M acetic acid. The retention times of tiazofurin and thymidine at an elution rate of 2.0 ml/minute were 8.7 minutes and 16 minutes respectively. The assay sensitivity was 1.0 μG/ml. The within run coefficient of variation of 2.0 μG/ml and 20 μG/ml plasma samples were ±7.49% and ±3.37% respectively and the between run coefficient of variation of 2.0 μG/ml and 20.0 μG/ml plasma samples were ±10.46% and ±5.92% respectively.Keywords
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