LIPID PEROXIDATION AND ITS INHIBITION BY TINORIDINE .2. ASCORBIC ACID-INDUCED LIPID PEROXIDATION OF RAT-LIVER MITOCHONDRIA

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  • 1 January 1979
    • journal article
    • research article
    • Vol. 572  (3) , 531-536
Abstract
Incubation of rat liver mitochondrial suspension with ascorbic acid and Fe2+ resulted in the formation of malondialdehyde and a decrease in the turbidity of the suspension. The maximum amount of malondialdehyde formed during the peroxidation reaction was about 1 mol/approximately 6 mol of mitochondrial phospholipids. Tinoridine may be and .alpha.-tocopherol at the concentration of 5 .mu.M and 1 mM respectively completely inhibited the peroxidative disintegration of mitochondria. The relationship between the concentration of tinoridine and the amount of malondialdehyde formed, showed that 1 mol of tinoridine prevents the formation of about 6 mol of malondialdehyde. There may be a limit in the chain reaction of the lipid peroxidation of mitochondria and the limit is the membrane sphere which is capable of releasing 6 molecules of malondialdehyde and contains about 36 molecules of the constitutive phospholipids.