Determination of Phosphatidylcholine Hydroperoxide in Human Plasma by Chemiluminescence - High Performance Liquid Chromatography
- 1 June 1988
- journal article
- research article
- Published by Taylor & Francis in Analytical Letters
- Vol. 21 (6) , 1033-1044
- https://doi.org/10.1080/00032718808071929
Abstract
To determine phosphatidylcholine hydroperoxide (PCOOH) in human plasma lipids, a chemiluminescence -high performance liquid chromatography (CL-HPLC) system was newly developed and applied. This system * To whom correspondence should be addressed. consisted of separation of phosphatidylcholine derivatives with normal phase HPLC and detection of hydroperoxide-specific chemiluminescence of PCOOH. By use of this system, the PCOOH in normal human plasma was quantitatively assayed to be 28-431 pmol hydroperoxide-02 per ml of plasma. The high sensitivities, reproducibilities and specificities of the CL-HPLC assay were shown for determining plasma PCOOH.Keywords
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