ASSAYING THE QUALITY OF CDNA LIBRARIES
- 1 April 1988
- journal article
- research article
- Vol. 6 (4) , 340-+
Abstract
Once a cDNA library has been constructed, it is very useful to be able to easily assess the quality of the library. Because actin is a ubiquitous sequence, this assessment has been accomplished by probing filter lifts and Southern blots of libraries with an actin cDNA probe. These methods provide information about the percent of actin-positive clones and the degree of completeness of cDNA clones in a library. Results of these methods are correlated with the success of finding full-length clones of interest.This publication has 18 references indexed in Scilit:
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