The induction of alkoxyresorufin metabolism: A potential indicator of environmental contamination

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Abstract
Methods of biochemical monitoring of individual animals for exposure to environmental contaminants are of great potential use. The hepatic metabolism of various alkoxyresorufins, which are highly specific substrates for certain forms of cytochrome(s) P450, is highly induced by a variety of environmental contaminants. Thus, theO-deal-kylation of pentoxy- or benzyloxyresorufin was induced greater than 20-fold in the rat bya-hexachlorocyclohexane, 2,4,5,2′,4′,5′-hexabromobiphenyl, DDT and Aroclor®-1254, while the metabolism of ethoxyresorufin was highly induced by 5,6-benzoflavone, 3,4,5,3′,4′,5′-hexabromobiphenyl and Aroclor®-1254. Additionally, rats exposed to diets containing as little as 12 ppm DDT displayed >five-fold increases in the rate of hepaticO-dealkylation of benzyloxyresorufin. Induction of the hepatic metabolism of these resorufin ethers in 9000xg supernatant fractions taken from rats exposed to potential environmental contaminants may constitute a valuable diagnostic indicator of the presence of a variety of pollutants, including poly cyclic aromatic hydrocarbons, organochlorine pesticides, polyhalogenated biphenyls and 2,3,7,8-tetrachlorodibenzo-p-dioxin. These results suggest the potential applicability of these substrates in detecting chemical contamination in the environment.

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