What's new: Ligation-based DNA diagnostics
- 1 November 1993
- Vol. 15 (11) , 761-765
- https://doi.org/10.1002/bies.950151112
Abstract
A number of novel gene detection techniques all revolve around the ligation of synthetic nucleic acid probes. In such ligase-assisted gene detection reactions, specific DNA or RNA sequences are investigated by using them as guides for the covalent joining of pairs of probe molecules. The probes are designed to hybridize immediately next to each other on the target nucleic acid strand. Demonstration of ligated probes results in highly specific detection of and efficient distinction between similar sequence variants under standard reaction conditions. Accordingly, the principle has been applied in automated genetic screening procedures. Ligation reactions are also integral to a number of amplification procedures and they will be of value in an expanding range of genetic analyses.Keywords
This publication has 23 references indexed in Scilit:
- A solid-support methodology for the construction of geometrical objects from DNAJournal of the American Chemical Society, 1992
- Molecular characterisation of a DNA ligase gene of the extremely thermophilic archaeonDesulfurolobus ambivalensshows close phylogenetic relationship to eukaryotic ligasesNucleic Acids Research, 1992
- Cloning, overexpression and nucleotide sequence of a thermostable DNA ligase-encoding geneGene, 1991
- Capture PCR: efficient amplification of DNA fragments adjacent to a known sequence in human and YAC DNA.Genome Research, 1991
- Chemical ligation of DNA: the first non-enzymatic assembly of a biologically active geneNucleic Acids Research, 1991
- Cloning, nucleotide sequence, and engineered expression of Thermus thermophilus DNA ligase, a homolog of Escherichia coli DNA ligaseJournal of Bacteriology, 1991
- The ligase chain reaction in a PCR world.Genome Research, 1991
- Synthesis from DNA of a molecule with the connectivity of a cubeNature, 1991
- Exponential amplification of nucleic acids: new diagnostics using DNA polymerases and RNA replicasesTrends in Biotechnology, 1991
- Structural and kinetic aspects of chemical reactions in DNA duplexes. Information on DNA local structure obtained from chemical ligation dataNucleic Acids Research, 1991