Evidence that Protein Synthesis can be Increased In Vitro Following Cholinergic Stimulation

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Abstract
These experiments were performed to evaluate secretory and synthetic responses of rat pancreas using in vitro experimental models. With previously described in vitro models, it was impossible to increase pancreatic protein synthesis over control level during or following stimulated enzyme secretion. An in vitro model is proposed combining four different incubation periods: preincubation, stimulation, stabilization, and incorporation. With this model, a 41% increase in protein synthesis has been observed following cholinergic stimulation. This increase occurred only if the previously increased secretion had returned to basal level during the incorporation period. These data would suggest that secretion and synthesis are two separate controlled mechanisms but any increase in synthesis requires a previously increased secretion.

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