Neonatal screening for 21-hydroxylase deficiency: a microfilter paper method for 17-α-hydroxyprogesterone assay

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Abstract
A micromethod for measuring 17a-hydroxyprogesterone in blo’od collected on filter paper has been developed. Our method is rapid, easy and has the specificity, accuracy and precisoin of the radioimmunoassay in whole blood. The method has been applied for screening patients with congenital adrenal hyperplasia (CAH) due to 21-hydroxylase deficiency. Fifty samples collected on filter paper were assayed by our method, using 1251 as tracer, and results were compared with those obtained for the same samples using a tritium tracer. The agreement between the two methods was particularly good in the area ranging from 15 to 100 pg/disc. In one neonate the diagnosis of CAH was made utilizing the microfilter paper method. Our method is a promising screening test for CAH. An indication of the advantages or disadvantages of this type of screening will become available when an adequate number of infants has been examined.