Identification of a novel HLA‐A allele (A*1115) in the UK National External Quality Assessment Schemes for Histocompatibility and Immunogenetics' Educational Cell Exchange

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Abstract
The novel allele, HLA‐A*1115, was identified in an ‘Educational Scheme’ sample (ED03/03 – from a north‐western European Caucasoid blood donor) distributed by the UK National External Quality Assessment Schemes for Histocompatibility and Immunogenetics. ED03/03 was typed by serology, the polymerase chain reaction using sequence‐specific primers and sequence‐based typing. A*1115 is most similar to A*110101 with a single mismatch (G to C) at constant position 565, leading to a conservative amino acid change from valine (GTG) to leucine (CTG) at codon 165 in the α2 domain. This substitution has not been reported for any other HLA class I allele so far. The HLA‐A*1115‐bearing haplotype was B*350101; Cw*040101; DRB1*140101; DRB3*020201; DQA1*010401; DQB1*0503; DPA1*0103/07; DPB1*030101. Extensive serological typing indicated that this allele essentially encodes a ‘normal’ HLA‐A11 specificity.