Effects of Arachidonic Acid and Other Unsaturated Fatty Acids on Mitogenesis in Human Lymphocytes
Open Access
- 1 April 1979
- journal article
- research article
- Published by Oxford University Press (OUP) in The Journal of Immunology
- Vol. 122 (4) , 1556-1562
- https://doi.org/10.4049/jimmunol.122.4.1556
Abstract
The effect of fatty acids and other lipids on mitogenic responses in cultured human peripheral blood lymphocytes was studied. Several-fold enhancement of tritiated thymidine incorporation was observed at 0.1 to 5.0 µg/ml concentrations of arachidonic acid. Other unsaturated fatty acids produced less marked changes. Increased responsiveness was demonstrable in a variety of media including RPMI 1640 supplemented with 10% fetal calf serum. Changes were also observed in uridine incorporation, total cell number, and blast transformation, indicating that the effect was not on thymidine transport or pool size per se. Arachidonic acid failed to affect PHA binding, indicating that the lectin-cell interaction was not being altered. Higher concentrations of fatty acids were inhibitory.This publication has 0 references indexed in Scilit: