Formation of Silver(I)-Mediated DNA Duplex and Triplex through an Alternative Base Pair of Pyridine Nucleobases

Abstract
We have recently reported the first artificial nucleoside for alternative DNA base pairing through metal complexation (J. Org. Chem.1999, 64, 5002−5003). In this context, we have accomplished a Ag(I)-mediated base pair or a base triplet in a double- or triple-stranded DNA, respectively, by introducing a pair of pyridine nucleobases in the middle of the sequence. As a result, the incorporated Ag(I) complex significantly stabilized the DNA duplex and triplex. This strategy would be expanded to the regulation of thermodynamic stability of DNA duplex or triplex by adding transition metal ions from outside, or to labeling applications in biotechnology.
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