A Toxicity Method with Calcium Cyclamate for Chronic Carcinogenesis Experiments

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Abstract
An experimental approach for determining the maximum tolerated dose of chemicals administered continuously or repeatedly ad libitum for the lifespan of animals is described. Five dose levels of calcium cyclamate were given in the drinking water for 35 days to Swiss mice, 8 animals in each group. By taking into account 4 parameters — survival rates, body weights, chemical consumption figures and histological changes — this method enables one to choose with certainty the proper dose, which is prerequisite to designing a chronic carcinogenesis experiment.