FRIGIDA-Independent Variation in Flowering Time of Natural Arabidopsis thaliana Accessions
Open Access
- 1 July 2005
- journal article
- research article
- Published by Oxford University Press (OUP) in Genetics
- Vol. 170 (3) , 1197-1207
- https://doi.org/10.1534/genetics.104.036533
Abstract
FRIGIDA (FRI) and FLOWERING LOCUS C (FLC) are two genes that, unless plants are vernalized, greatly delay flowering time in Arabidopsis thaliana. Natural loss-of-function mutations in FRI cause the early flowering growth habits of many A. thaliana accessions. To quantify the variation among wild accessions due to FRI, and to identify additional genetic loci in wild accessions that influence flowering time, we surveyed the flowering times of 145 accessions in long-day photoperiods, with and without a 30-day vernalization treatment, and genotyped them for two common natural lesions in FRI. FRI is disrupted in at least 84 of the accessions, accounting for only ∼40% of the flowering-time variation in long days. During efforts to dissect the causes for variation that are independent of known dysfunctional FRI alleles, we found new loss-of-function alleles in FLC, as well as late-flowering alleles that do not map to FRI or FLC. An FLC nonsense mutation was found in the early flowering Van-0 accession, which has otherwise functional FRI. In contrast, Lz-0 flowers late because of high levels of FLC expression, even though it has a deletion in FRI. Finally, eXtreme array mapping identified genomic regions linked to the vernalization-independent, late-flowering habit of Bur-0, which has an alternatively spliced FLC allele that behaves as a null allele.Keywords
This publication has 45 references indexed in Scilit:
- Light-Response Quantitative Trait Loci Identified with Composite Interval and eXtreme Array Mapping in Arabidopsis thalianaSequence data from this article have been deposited with the EMBL/GenBank Data Libraries under accession nos. AY394847 and AY466496.Genetics, 2004
- A New Arabidopsis Gene,FLK, Encodes an RNA Binding Protein with K Homology Motifs and Regulates Flowering Time viaFLOWERING LOCUS C [W]Plant Cell, 2004
- Analysis of the Molecular Basis of Flowering Time Variation in Arabidopsis AccessionsPlant Physiology, 2003
- Large-Scale Identification of Single-Feature Polymorphisms in Complex GenomesGenome Research, 2003
- Nonsense-Associated Altered SplicingMolecular Cell, 2002
- Climatic adaptation of bud set and frost hardiness in Scots pine (Pinus sylvestris)Canadian Journal of Forest Research, 1997
- Climatic adaptation of bud set and frost hardiness in Scots pine (Pinus sylvestris)Canadian Journal of Forest Research, 1997
- The phenotype of some late‐flowering mutants is enhanced by a locus on chromosome 5 that is not effective in the Landsberg erecta wild‐typeThe Plant Journal, 1994
- Isolation of LUMINIDEPENDENS: a gene involved in the control of flowering time in Arabidopsis.Plant Cell, 1994
- MAPMAKER: An interactive computer package for constructing primary genetic linkage maps of experimental and natural populationsGenomics, 1987