Simultaneous Quantitation of Prostaglandins F 1? . (PGF 1? ) and PGF 2aL by Selected Ion Recording with a Deuterated Internal Standard

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Abstract
A gas chromatographic-mass spectrometric method for the simultaneous measurement of PGF1α, and PGF2α at the ng level is reported. The internal standard was [3, 3, 4, 4-D] PGF2α. The method involves conversion of the substrates to their methyl ester-trimethyl-silyl ether (ME-TMS) derivatives and selected ion recording. The standard curves were reproducible and linear between 0. 5 and 40 ng (1. 4 to 112 picomole). The regression characteristics of the standard curves for each PGF were virtually identical to those of the analogous curves from separate determinations.