A SENSITIVE RADIOIMMUNOASSAY FOR BOVINE ANTI-MŰML;LLERIAN HORMONE, ALLOWING ITS DETECTION IN MALE AND FREEMARTIN FETAL SERUM

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Abstract
Two monoclonal antibodies have been used to set up a solid-phase RIA for bovine anti-Milllerian hormone (bAMH). One AMH unit is defined as the amount released by 1 g of bovine fetal testicular tissue during a 4 h incubation period. Calibration curves were prepared using aliquots of a standard 500 ml pool of incubation medium, containing 200 AMH mU/ml, diluted either in 50 % pig testes incubation medium, 5 % horse serum, 10 % female calf fetal serum or pure female calf fetal serum. Linearization of the calibration curves was achieved through “logit-log” transformation, all four lines were parallel. Within and between-assay variability were less than 5 %. The RIA is at least 600 times more sensitive than the bioassay for anti- Miillerian activity and can detect AMH in male and freemartin fetal serum.