Immunochemical delineation of an oncofetal antigen on normal and simian virus 40-transformed human fetal melanocytes.

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Abstract
Human melanoma cells of uveal origin shed 94,000- and 240,000-dalton glycoproteins in common with most melanoma cell lines of dermal origin. Normal human melanocytes derived from fetal uvea shed a 90,000-dalton glycoprotein that was immunologically identical with the 94,000-dalton glycoprotein of melanoma cells. Expression of this 90,000-dalton molecule was confined to fetal cells of ectodermal origin. After SV40 transformation of human fetal melanocytes, there was an apparent increase in molecular size of this component to 94,000 daltons. The 240,000-dalton glycoprotein was not synthesized or shed by uninfected or SV40-transformed fetal melanocytes. The 94,000-dalton glycoprotein is apparently an oncofetal antigen of ectodermal origin.