EFFECT OF ALBUMIN BINDING ON THE HEPATIC TRANSPORT OF ROSE-BENGAL - SURFACE-MEDIATED DISSOCIATION OF LIMITED CAPACITY

  • 1 January 1982
    • journal article
    • research article
    • Vol. 223  (2) , 342-347
Abstract
The steady-state removal of Rose Bengal by perfused rat livers was studied to decide if the tight association of this anion with albumin is consistent with the conventional view that only the free fraction in the sinusoidal lumen is available for hepatic transport. The concentration of free dye is not nearly high enough to account for the observed removal rate. The liver cell surface reduces the affinity of Rose Bengal for albumin by a factor of .apprx. 100 below that observed in free solution. The findings extend and refine the results of a previous experiment with taurocholate. The dissociation mechanism displays saturation kinetics as the albumin concentration increases.