A new selective medium for isolating Listeria spp. from heavily contaminated material
- 1 January 1988
- journal article
- research article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 54 (1) , 165-167
- https://doi.org/10.1128/aem.54.1.165-167.1988
Abstract
Food-associated outbreaks of human listeriosis have emphasized the importance and necessity of screening food for the presence of Listeria isolates-selective agar medium combining acriflavine (10 mg/liter) with ceftazidime (50 mg/liter) was developed. A total of 1,099 cheese production specimens were cultured, from which 157 Listeria isolates. (14.3%) grew. When compared with modified McBride agar, the acriflavine-ceftazidime agar recovered more Listeria isolates (98 versus 65%, P less than 0.001) more rapidly (57% after 48 h of incubation of the enrichment broth versus 35%, P less than 0.01) and in greater amounts. Acriflavine-ceftazidime selective agar medium proved to be a highly sensitive medium to recover Listeria spp. from heavily contaminated food products.This publication has 6 references indexed in Scilit:
- Isolation of Listeria monocytogenes from raw milkApplied and Environmental Microbiology, 1986
- Pasteurized Milk as a Vehicle of Infection in an Outbreak of ListeriosisNew England Journal of Medicine, 1985
- New methodology for the isolation of Listeria microorganisms from heavily contaminated environmentsApplied and Environmental Microbiology, 1984
- Epidemic Listeriosis — Evidence for Transmission by FoodNew England Journal of Medicine, 1983
- A Method for the Rapid Isolation ofListeria monocytogenesfrom Infected MaterialJournal of Applied Bacteriology, 1977