The determination of uric acid in biological materials

Publisher Website
Google Scholar
Abstract
In the reaction between uric acid and phosphotungetic acid in the presence of NaCN, the effects of time, temperature, concentration of reactants and alkalinity of the reaction mixture were investigated. The method of Folin for the determination of uric acid was modified as follows Tungstic acid filtrate (0.2 ml), 4% (w/v) NaCN containing 15% (w/v) of urea (3 ml) and Folin''s reagent (0.5 ml) are mixed and heated at 35[degree] for 30 minutes. The solution is cooled and the optical density determined at 690-700 mu. Duplicate tests agree within 0.12 mg/100 ml of serum and recoveries are 96.8% (S.D. 6.3). The method can be applied to urine.

This publication has 3 references indexed in Scilit: