On the quantitative determination of adenine in the presence of guanine
- 1 January 1934
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 28 (6) , 2097-2101
- https://doi.org/10.1042/bj0282097
Abstract
The method generally used in separating adenine from guanine by their different solubilities in dilute ammonia is unreliable because adenine is not soluble enough in neutral or weakly alkaline solns. to allow a quantitative separation. The author therefore detd. the total purine bases (in yeast nucleic acid) before and after destruction of guanine by K permanganate in acid solution, which leaves adenine intact.This publication has 1 reference indexed in Scilit:
- Mitbestimmungen von Purinsubstanzen in Geweben. 2. Mitteilung. Die Bestimmung des Guanins, des Adenins und der Oxypurine.Hoppe-Seyler´s Zeitschrift Für Physiologische Chemie, 1933