The Use of a Radiometric Technic for the Rapid Detection of Contaminated Tissue Specimens From Burned Patients

Abstract
The 14CO2-dependent BACTEC® system for the detection of bacteria in fluid specimens was adapted for the semiquantitative bacteriologic screening of solid tissue samples from 33 burned patients. The radiometric system detected 87% of all specimens harboring > 105 organisms per gram of tissue and 100% of all heavily contaminated specimens (> 108 organisms per gram) within eight hours of incubation. The BACTEC radiometric system correlated well (98.1%) with conventional culture methods in the detection of all contaminated samples. No false-positive result was obtained from sterile tissue specimens.

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