SIGNIFICANCE OF THE LOW-DENSITY LIPOPROTEIN (LDL) RECEPTOR PATHWAY FOR THE INVITRO ACCUMULATION OF AD-32 INCORPORATED INTO LDL IN NORMAL AND LEUKEMIC WHITE BLOOD-CELLS

  • 1 January 1984
    • journal article
    • research article
    • Vol. 68  (3) , 515-520
Abstract
The accumulation of the lipophilic cancer chemotherapeutic agent AD-32 as free drug and incorporated into low-density lipoprotein (LDL) (AD-32-LDL) was studied in wbc [white blood cells] from normal subjects and from patients with acute myelogenous leukemia exhibiting high-affinity uptake and degradation of 125I-LDL. The rate of degradation of 125I-LDL in the leukemic cells was 4-25 times higher than that in normal wbc. The accumulation of LDL-incorporated AD-32 was greater than that of the free drug and could be reduced by the addition of native LDL in excess. AD-32-LDL was as effective as native LDL in inhibiting the degradation of 125I-LDL. Preincubation of wbc in a lipoprotein-deficient medium enhanced both the degradation of 125I-LDL and the cellular drug accumulation on incubation with AD-32-LDL. Heparin inhibited both the high-affinity degradation of 125I-LDL and the cellular accumulation of LDL-incorporated drug. However, comparison of the high-affinity degradation rate of 125I-LDL and the accumulation of LDL-incorporated AD-32 showed that the cellular drug accumulation markedly exceeded that which could be explained by high-affinity uptake and degradation of AD-32-LDL. Furthermore, saturation of the cellular uptake of LDL-incorporated drug occurred at higher LDL concentrations than saturation of 125I-LDL degradation. Cellular uptake of AD-32-LDL may be partly mediated by the LDL receptor pathway and partly by a nonspecific mechanism.

This publication has 0 references indexed in Scilit: