Interaction of calmodulin with lactoferrin
- 24 February 1992
- journal article
- Published by Wiley in FEBS Letters
- Vol. 298 (2-3) , 195-198
- https://doi.org/10.1016/0014-5793(92)80055-l
Abstract
Calmodulin, as a major intracellular calcium-binding protein, regulates many Ca2+-dependent enzymes and plays an important role in a wide spectrum of cellular functions of the eukaryotes. Interaction between calmodulin and human lactoferrin, a 78 kDa protein with antibacterial properties, was found in the presence of Ca2+ using (i) a method for the detection of calmodulin binding proteins with biotinylated calmodulin, (ii) affinity chromatography on an agarose—calmodulin column with subsequent detection by an enzyme-linked immunosorbent assay (ELISA). The binding of calmodulin to lactoferrin blocked the ability of lactoferrin to agglutinate Micrococcus lysodeikticus.Keywords
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