Matrix metalloproteinase-9 expression in mammary gland tumors in dogs and its relationship with prognostic factors and patient outcome
- 1 May 2012
- journal article
- Published by American Veterinary Medical Association (AVMA) in American Journal of Veterinary Research
- Vol. 73 (5) , 689-697
- https://doi.org/10.2460/ajvr.73.5.689
Abstract
Objective—To immunohistochemically evaluate matrix metalloproteinase (MMP)-9 expression in benign and malignant mammary gland tumors (MMTs) in dogs and relate expression to prognostic factors and patient outcome. Animals—118 female dogs with naturally occurring mammary gland tumors and 8 dogs without mammary gland tumors. Procedures—24 benign mammary gland tumors and 94 MMTs (1/affected dog) were obtained during surgical treatment; control mammary gland tissue samples were collected from unaffected dogs after euthanasia for reasons unrelated to the study. Tumors were evaluated for proliferation, invasive growth, histologic grade, and metastatic capacity; expression of MMP-9 was determined immunohistochemically, and its relationship with clinical and histologic findings was investigated. For dogs with MMTs, follow-up continued for 2 years; data were used to compute overall survival time and disease-free interval and construct survival curves. Results—MMTs had significantly higher MMP-9 expression in stromal cells and in neo-plastic cells than did the benign neoplasms. Stromal MMP-9 expression was also higher in highly proliferative tumors and in tumors with invasive growth, high histologic grade, and metastatic capacity. Furthermore, tumors from patients with shorter overall survival times and disease-free intervals had higher expression of MMP-9 in stromal cells. Conclusions and Clinical Relevance—In dogs with MMTs, level of MMP-9 expression by stromal cells was related to factors of poor prognosis and shorter overall survival times and disease-free intervals. These results suggested that MMP-9 produced by tumor-adjacent stromal cells contributed to MMT progression in female dogs and that assessment of MMP-9 expression may be a valuable prognostic factor.Keywords
This publication has 29 references indexed in Scilit:
- Expression and prognostic significance of metalloproteases and their inhibitors in luminal A and basal-like phenotypes of breast carcinomaHuman Pathology, 2009
- Comparative analysis and clinical value of the expression of metalloproteases and their inhibitors by intratumor stromal fibroblasts and those at the invasive front of breast carcinomasBreast Cancer Research and Treatment, 2009
- Study of matrix metalloproteinases and their inhibitors in breast cancerBritish Journal of Cancer, 2007
- Matrix metalloproteinases: old dogs with new tricksGenome Biology, 2003
- New insights into the role of extracellular matrix during tumor onset and progressionJournal of Cellular Physiology, 2002
- MMP-2 and MMP-9 Expression in Breast Cancer-Derived Human Fibroblasts is Differentially Regulated by Stromal-Epithelial InteractionsBreast Cancer Research and Treatment, 2002
- High expression of 92 kDa type IV collagenase (matrix metalloproteinase-9) in canine mammary adenocarcinomaBiochimica et Biophysica Acta (BBA) - General Subjects, 2001
- How Matrix Metalloproteinases Regulate Cell BehaviorAnnual Review of Cell and Developmental Biology, 2001
- Metalloproteinases: role in breast carcinogenesis, invasion and metastasisBreast Cancer Research, 2000
- Identification of matrix metalloproteinases in canine neoplastic tissueAmerican Journal of Veterinary Research, 2000