IMPAIRMENT OF ANTIGEN-PRESENTING CELL FUNCTION BY ULTRAVIOLET RADIATION II. EFFECT OF IN VITRO ULTRAVIOLET IRRADIATION ON ANTIGEN-PRESENTING CELLS

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Abstract
The s.c. injection of 10 mM 2,4,6-trinitrobenzene sulfonic acid (TNBS) derivatized splenic adherent cells (SAC) into syngeneic mice primes for contact sensitivity or delayed-type hypersensitivity (DTH) when these animals are challenged with picryl chloride on the ear or trinitrophenol(TNP)-coupled cells in the footpad, respectively. If recipient mice are exposed to UV light irradiation and are immunized with normal TNP-treated SAC, they develop marked DTH reaction upon challenge but develop limited DTH reactions if immunized with hapten-derivatized SAC that had been obtained from UV-treated recipients. If the SAC are obtained from normal mice but are treated in vitro with UV light (1.2-1.4 mJ/cm2 per s over the wavelength range 280-340 nm at a tube to target distance of 20 cm), these cells can neither prime nor elicit hapten-specific T cell immunity in UV-treated recipients. If UV-treated TNP SAC are used to prime UV-irradiated recipients, TNP-specific suppressor T cells are generated rather than T effector cells.