The Effect of Protriptyline on Uptake and Retention of dl‐3H‐Nor adrenaline in Different Tissues of the Rat

Publisher Website
Google Scholar
Abstract
The inhibition of3H‐noradrenaline (3H‐NA) uptake in submaxillary glands, spleens and heartr of the rat was studied after pretreatment of the animals with different doses of protriptyline (PTP). One μg/kg3H‐NA was administered intravenously 15 min after the intraperitoneal injection of PTP. The duration of the uptake blockade induced by 10 mg/kg PTP was also studied. To circumvent problems due to the blood flow, anin vitrotechnique was also used to compare the uptake and retention of3H‐NA in submaxillary glands and hearts. It was found that the3H‐NA uptake of the heartin vivonormally exceeds several times that of the submrtvillary glands, whilein vitrothe uptake was directly correlated to the concentration of endogenous NA. The data support the view that the greater blood flow through the heart is responsible for the major part of the difference foundin vivo. After PTP pretreatment the uptake inhibition was much stronger in the heart than in the other organs investigatedin vivo. Alsoin vitrothere was a more efficient inhibition of the3H‐NA uptake in the heart than in the submaxillary gland, both when 10 mg/kg PTP was given i.p. lo the animals 30 min prior to death and when 1μg/ml of the drug was present in the incubation medium. The factors deter mining the overall sensitivity of different organs to PTP are discussed.