Growth of Philophthalmus sp. (Trematoda) on the Chorioallantois of the Chick

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Abstract
Although previous attempts to grow or even maintain larval trematodes on the chorio-allantoic membrane of the chick have been unsuccessful, growth and development of Philophthalmus sp., an ocular trematode, on the chorioallantois have now been achieved, indicating basic similarities in this site and the nictitating membrane. The chorioallantoic membranes of 49 7- to 10-day-old chick embryos, were exposed to 3 to 50 oligoseptic metacercariae each, and examined 1 to 8 days later. Twenty-two embryos contained 8.3% of the inoculated larvae with growth parallelling that of the normal site. Serial transfers were conducted at 7-day intervals. Successful transfers were recovered on days 14 and 20. Beyond the 7th day the growth rate lagged about 25% below normal. Specimens became ovigerous past the 14th day but before the 20th, compared to 13 days in vivo. Chorioallantoic-flukes, although showing a lag in development when compared to specimens grown in chicks'' eyes, reached four of the five developmental stages.

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