Biochemical studies on the use of sodium hyaluronate in the anterior eye segment. I. Variation of protein and ascorbic acid concentration in rabbit aqueous humor

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Abstract
To investigate the safety of the use of sodium hyaluronate in anterior segment surgery, the changes in protein, ascorbic acid and hyaluronate concentration in the aqueous humor, and intraocular pressure were followed after sodium hyaluronate was injected into the anterior chamber. When a 1% solution of sodium hyaluronate with a molecular weight of 800K was injected, the recovery patterns of protein and ascorbic acid concentration to normal levels were the same as the recovery patterns after injection of phosphate buffered saline which was the solvent for sodium hyaluronate. However, when a 1% solution of sodium hyaluronate with a molecular weight of 1640K or a 2% solution with a molecular weight of 800K was injected, it was observed that the recovery times of protein and ascorbic acid concentration to normal levels were longer than that required after injection of 1%,800K sodium hyaluronate. Further, the recovery time of the aqueous humor components to normal levels was associated with the clearance time of sodium hyaluronate from the anterior chamber. The transient increase in intraocular pressure by injection of sodium hyaluronate was observed, but it disappeared in 12 hrs. The recovery time of intraocular pressure to normal level was independent of molecular weight and concentration of sodium hyaluronate tested.