Quantitation of the growth‐associated protein B‐50/GAP‐43 and neurite outgrowth in PC12 cells

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Abstract
A combined assay to measure neurite outgrowth and B-50/GAP-43 levels in PC 12 cells is reported. During NGF-induced neuritogenesis, B-50/GAP-43 expression was monitored by enzyme-linked immunosorbent assay (ELISA). Neurite outgrowth was quantified at the same time by the use of video image analysis. Sensitivity and reliability of the methods are shown with a dose–response and time curve of β-NGF-induced neuritogenesis. A linear increase in total length of neurites was induced by concentrations of β-NGF ≥5 ng/ml and was accompanied by a linear increase in the amount of B-50/GAP-43. The combined methods presented here can conveniently and reliably establish subtle changes in neurite outgrowth and intracellular protein contents.