Prostate cell lines secrete IL‐8 in response to mycoplasma hominis through Toll‐like receptor 2‐mediated mechanism

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Abstract
Background The roles of Toll‐like receptor 2 (TLR2) and Mycoplasma in prostate inflammation remain unclear. We investigated whether Mycoplasma induced inflammatory cytokine secretion through TLR2‐mediated mechanism in prostate cancer cell line. Methods Cell surface expression of TLR2 on PC‐3 cells were examined by flowcytometry. PC‐3 cells were stimulated with Mycoplasma hominis (M. hominis), and IL‐8 secretion and NF‐κB activation were examined. Results PC‐3 cells expressed TLR2 mRNA and cell surface TLR2 protein. The membrane fraction of M. hominis induced IL‐8 secretion from PC‐3 cells and NF‐κB activation in a concentration‐dependent manner. Transient transfection of the dominant negative mutant TLR2P681H into PC‐3 cells attenuated M. hominis‐induced IL‐8 secretion and NF‐κB activation. Antibody against the extracellular TLR2 domain significantly suppressed M. homnis‐induced IL‐8 secretion from the prostate cell lines including PC‐3, PrEC, and transformed myofibroblasts. Conclusions These results clearly demonstrate that the prostate cell line can secrete inflammatory cytokine in response to M. hominis through a TLR2‐mediated mechanism.

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