Studies in the metabolism of the strict anaerobes (genus Clostridium)

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Abstract
Washed suspensions of Cl. sporogenes activate certain amino-acids as H-donators (especially alanine, valine and leucine) and others as: H-acceptors (glycine, proline and hydroxyproline). 1 molecule of alanine reduces 2 of cresyl blue; the amino-acid is deaminated during the first step of oxidation. Direct reactions occur between the H-donating and the H-accepting amino-acids, any one of one group reacting with any one of the other. Reaction rates are of the same order as those of aerobic oxidations with other bacteria, e.g., Q02alanine + proline = 50 Whether reactions of this hitherto unknown type are important quantitatively in providing energy for growth on protein media has not yet been detd. experimentally, but there can be little doubt that they are.

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