A method for recovering enterovirus from infected samples by adsorption onto shaken suspended cells

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Abstract
A method is described whereby enterovirus present in a 5-ml liquid sample may be efficiently recovered for plaque enumeration on a single 60 × 15 mm culture dish. Adsorption is accomplished by constant agitation of the sample with suspended cells followed by a stationary period to allow monolayer formation for plaque development. Comparative studies with the use of coxsackievirus B5 and poliovirus 1, on HEp-2 and Vero cells respectively, indicated that the conventional method of adsorption onto stationary monolayers would require 50 times as many dishes and cells for an equal efficiency. The new method might be useful for the recovery of small numbers of viruses from food or water samples providing they are nontoxic to cells and contain no bacteria.

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