In vitroculture of raspberry (Rubus ideaus) for mass propagation
- 1 January 1985
- journal article
- research article
- Published by Taylor & Francis in The Journal of Horticultural Science and Biotechnology
- Vol. 60 (4) , 493-499
- https://doi.org/10.1080/14620316.1985.11515656
Abstract
Summary In a method for the rapid micropropagation of six raspberry cultivars the growth of meristems was improved if the concentration of NH4NO3 and KNO3 in Murashige and Skoog (MS) medium was reduced to half strength. Sufficient numbers of axillary shoots were produced using a modified MS medium supplemented with 1.0 mg 1−1 BAP, provided both shoot tips and nodal segments were used. A very poor proliferation rate was obtained from only shoot tips. An intervening elongation medium with the addition of GA between shoot proliferation and rooting was not effective. Different GA concentrations had little effect on stem elongation and inhibited subsequent rooting. Four of the cultivars gave 100% rooting if the macronutrients in MS medium were reduced to one fifth plus 0.01 mg l−1 IBA and dark treatment for the first 5 days in the rooting medium. After transfer to soil ≥95% of rooted plants survived. Two of the cultivars gave c. 90% survived.This publication has 0 references indexed in Scilit: