The DNA content of human plasma cells
- 15 May 1981
- Vol. 47 (10) , 2433-2436
- https://doi.org/10.1002/1097-0142(19810515)47:10<2433::aid-cncr2820471020>3.0.co;2-d
Abstract
The DNA content of human plasma cells from myeloma patients relative to that of leukocytes was determined by flow and microscopic cytofluorometry after propidium iodide and fluorescent Feulgen staining, respectively. Mononucleated myeloma plasma cells from all of the 17 patients studied contained more DNA (17 to 58%) than the leukocytes from the same patient. The binucleated and trinucleated plasma cells, which were more prevalent in advanced cases, contained up to two and three times, respectively, the amount of DNA determined in the mononucleated plasma cells. These observations suggest that the ploidy abnormalities of myeloma plasma cells are even more extensive than the numerous karyotypic studies have indicated.This publication has 6 references indexed in Scilit:
- Karyotypic abnormalities and clinical aspects of patients with multiple myeloma and related paraproteinemic disordersCancer, 1979
- Rapid flow cytofluorometric analysis of mammalian cell cycle by propidium iodide staining.The Journal of cell biology, 1975
- Effect of chemotherapy on the labelling index of myeloma cellsCancer, 1974
- A simple procedure for the separation of viable blood cells, suitable for long-term in vitro experimentsBiochemical Medicine, 1974
- Cytogenetic Studies in Multiple Myeloma 2JNCI Journal of the National Cancer Institute, 1974
- Separation of cells by velocity sedimentationJournal of Cellular Physiology, 1969