PCR‐linked in vitro expression: a novel system for high‐throughput construction and screening of protein libraries
Open Access
- 14 March 2003
- journal article
- Published by Wiley in FEBS Letters
- Vol. 540 (1-3) , 147-150
- https://doi.org/10.1016/s0014-5793(03)00251-5
Abstract
A novel entirely in vitro strategy for generation and screening of a combinatorial protein library in an array format has been developed and is experimentally demonstrated. The strategy exploits virtues of PCR and in vitro coupled transcription/translation. Our new approach provides high‐throughput construction and screening of the in vitro protein library, and compatibility with various selection methods.Keywords
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