Export-Mediated Assembly of Mycobacterial Glycoproteins Parallels Eukaryotic Pathways
Top Cited Papers
- 5 August 2005
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 309 (5736) , 941-943
- https://doi.org/10.1126/science.1114347
Abstract
Protein O-mannosylation is an essential and evolutionarily conserved post-translational modification among eukaryotes. This form of protein modification is also described in Mycobacterium tuberculosis; however, the mechanism of mannoprotein assembly remains unclear. Evaluation of differentially translocated chimeric proteins and mass spectrometry to monitor glycosylation demonstrated that specific translocation processes were required for protein O-mannosylation in M. tuberculosis. Additionally, Rv1002c, a M. tuberculosis membrane protein homolog of eukaryotic protein mannosyltransferases, was shown to catalyze the initial step of protein mannosylation. Thus, the process of protein mannosylation is conserved between M. tuberculosis and eukaryotic organisms.Keywords
This publication has 25 references indexed in Scilit:
- The Streptococcus gordonii Platelet BindingProtein GspB Undergoes Glycosylation Independently ofExportJournal of Bacteriology, 2004
- The primary mechanism of attenuation of bacillus Calmette–Guérin is a loss of secreted lytic function required for invasion of lung interstitial tissueProceedings of the National Academy of Sciences, 2003
- The MPB83 Antigen from Mycobacterium bovis ContainsO-Linked Mannose and (1 → 3)-Mannobiose MoietiesPublished by Elsevier ,2003
- Bacterial glycoproteins: Functions, biosynthesis and applicationsProteomics, 2003
- N-Linked Glycosylation in Campylobacter jejuni and Its Functional Transfer into E. coliScience, 2002
- TheneuA/flmDgene cluster ofHelicobacter pyloriis involved in flagellar biosynthesis and flagellin glycosylationFEMS Microbiology Letters, 2002
- Structure-Function Analysis of the Dolichyl Phosphate-Mannose: Protein O-Mannosyltransferase ScPmt1pJournal of Biological Chemistry, 2000
- Host Defense Mechanisms Triggered by Microbial Lipoproteins Through Toll-Like ReceptorsScience, 1999
- Protein O-mannosylationBiochimica et Biophysica Acta (BBA) - General Subjects, 1999
- TARGETING AND ASSEMBLY OF PERIPLASMIC AND OUTER-MEMBRANE PROTEINS IN ESCHERICHIA COLIAnnual Review of Genetics, 1998