Evaluating Neutralizing Antibodies Against HIV, SIV, and SHIV in Luciferase Reporter Gene Assays
Top Cited Papers
Open Access
- 1 December 2004
- journal article
- unit
- Published by Wiley in Current Protocols in Immunology
- Vol. 64 (1) , 12.11.1-12.11.17
- https://doi.org/10.1002/0471142735.im1211s64
Abstract
Neutralizing antibody assays are powerful tools for assessing humoral immunity in AIDS virus infection and vaccine development. Although many different assays have been described, all are based on the same principle, measuring reductions in virus infectivity. This unit describes two assays utilizing a genetically engineered cell lines that are susceptible to infection by most strains of HIV‐1, SIV, and SHIV. One assay is designed for optimal performance with uncloned viruses produced in either PBMC or CD4+ T cell lines. A second assay is designed for single‐cycle infection with molecularly cloned pseudoviruses produced by transfection in 293T cells. Both assays are performed in a 96‐well format and use tat‐responsive luciferase reporter gene expression as readout.Keywords
This publication has 33 references indexed in Scilit:
- Antibody neutralization and escape by HIV-1Nature, 2003
- Human Immunodeficiency Virus Type 1 Neutralization Measured by Flow Cytometric Quantitation of Single-Round Infection of Primary Human T CellsJournal of Virology, 2002
- Prospects for Vaccine Protection Against HIV-1 Infection and AIDSAnnual Review of Immunology, 2002
- A Luciferase-Reporter Gene-Expressing T-Cell Line Facilitates Neutralization and Drug-Sensitivity Assays That Use Either R5 or X4 Strains of Human Immunodeficiency Virus Type 1Virology, 2001
- Immunization with Recombinant Canarypox Vectors Expressing Membrane-Anchored Glycoprotein 120 Followed by Glycoprotein 160 Boosting Fails to Generate Antibodies That Neutralize R5 Primary Isolates of Human Immunodeficiency Virus Type 1AIDS Research and Human Retroviruses, 2000
- CHEMOKINE RECEPTORS AS HIV-1 CORECEPTORS: Roles in Viral Entry, Tropism, and DiseaseAnnual Review of Immunology, 1999
- G protein-coupled receptors in HIV and SIV entry: New perspectives on lentivirus–host interactions and on the utility of animal modelsSeminars in Immunology, 1998
- Antigen Detection in Neutralization Assays: High Levels of Interfering Anti-p24 Antibodies in Some PlasmaAIDS Research and Human Retroviruses, 1993
- International Collaborative Study to Compare Assays for Antibodies that Neutralize Human Immunodeficiency VirusAIDS Research and Human Retroviruses, 1990
- Simple, Rapid, Quantitative, Syncytium-Forming Microassay for the Detection of Human Immunodeficiency Virus Neutralizing AntibodyAIDS Research and Human Retroviruses, 1987