Hormones in Plants Bearing Nitrogen-fixing Root Nodules: Partial Characterisation of Cytokinins from Root Nodules ofAlnus glutinosa(L.) Gaertn

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Abstract
The nature of the substances responsible for the major cytokinin activity in extracts of Alnus glutinosa (L.) Gaertn. root nodules was investigated by means of chromatographic, chemical, and enzymic methods. Five cytokinins were demonstrated and a further two compounds were probably present in trace amounts. The properties of the cytokinins were consistent with their being identical or closely similar to trans-zeatin, trans-zeatin riboside, zeatin-O-β-D-glucoside, and a β-D -glucoside of zeatin riboside together with certain of the corresponding dihydrozeatin compounds. The greatest part of the cytokinin activity was represented by the glucosides.

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