THE PREPARATION OF CRYSTALLINE TRANSALDOLASE FROM CANDIDA UTILIS

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Abstract
Transaldolase forms a stable intermediate complex with dihydroxyacetone in the process of transferring this group from substrate to acceptor. The formation of such a stable complex provides a valuable approach to study of the mechanism of action of the aldolase-type enzyme. It is essential, however, that the enzyme be available in pure form and in large quantities. The procedure reported here makes this possible. It requires only a few operations and employs no chromatographic procedures. The overall yield is nearly 25% and the product is highly stable and free of other enzymes. It thus offers a number of advantages over the procedure reported by Venkataraman and Racker.