Coat Protein is Required for Infection of Cowpea Protoplasts with Alfalfa Mosaic Virus
Open Access
- 1 December 1978
- journal article
- Published by Microbiology Society in Journal of General Virology
- Vol. 41 (3) , 653-656
- https://doi.org/10.1099/0022-1317-41-3-653
Abstract
Infection of cowpea protoplasts with alfalfa mosaic virus RNA was achieved by sedimenting protoplasts from 0.5 m-mannitol and resuspending them in 0.7 m-mannitol containing 0.1 m-potassium phosphate, pH 6.0, and 100 µg/ml RNA. The osmotic shift-up employed during inoculation substantially enhanced the infection by virus RNA as well as by virus nucleoprotein.Keywords
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