Microsatellite Markers Linked to Salmonella enterica Serovar Enteritidis Vaccine Response in Young F1 Broiler-Cross Chicks

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Abstract
Reduction in Salmonella enteritidis (SE) contamination is of importance for poultry production as well as for food safety. The objectives of this study were to identify potential genetic markers of antibody response to SE vaccine in young broiler chicks and then to confirm this linkage in broiler-cross offspring, as well as to explore interactions of marker alleles with dam line and sex. The initial identification of suggestive quantitative trait loci (QTL) markers for antibody response to SE vaccine was conducted by using bulked segregant analysis (BSA) with 58 microsatellite markers in a broiler breeder male line. Four unlinked microsatellites that had allele frequency differences between the high and low antibody response DNA pools were selected for subsequent analysis in a linkage study. Antibody response was measured in an F1 population (n = 379) that was derived by crossing each of four males of the broiler line with several dams from four genetically distant, highly inbred lines (Spanish, Fayoumi, and MHC-congenic G-B1 and G-B2 Leghorn). These crosses enabled us to evaluate the broiler sire QTL-marker allele effects and to explore QTL interactions with the dam lines by individual genotyping. Each of the four microsatellites identified by BSA in the broiler population had a significant (P < 0.05) association with F1 population antibody response in one or more sire families. The effect of the interaction of microsatellite allele with dam line or sex on antibody response was frequently significant. Microsatellite markers linked to antibody response QTL were identified, and genetic interactions with dam line and sex were detected.

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