Heat-Stable Proteins and Abscisic Acid Action in Barley Aleurone Cells
- 1 December 1989
- journal article
- research article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 91 (4) , 1520-1526
- https://doi.org/10.1104/pp.91.4.1520
Abstract
[35S]Methionine labeling experiments showed that abscisic acid (ABA) induced the synthesis of at least 25 polypeptides in mature barley (Hordeum vulgare) aleurone cells. The polypeptides were not secreted. Whereas most of the proteins extracted from aleurone cells were coagulated by heating to 100.degree. C for 10 minutes, most of the ABA-induced polypeptides remained in solution (heat-stable). ABA had little effect on the spectrum of polypeptides that were synthesized and secreted by aleurone cells, and most of these secreted polypeptides were also heat-stable. Coomassie blue staining of sodium dodecyl sulfate polyacrylamide gels indicated that ABA-induced polypeptides already occurred in high amounts in mature aleurone layers having accumulated during grain development. About 60% of the total protein extracted from mature aleurone was heat stable. Amino acid analyses of total preparations of heat-stable and heat-labile proteins showed that, compared to heat-labile proteins, heat-stable intracellular proteins were characterized by higher glutamic acid/glutamine (Glx) and glycine levels and lower levels of neutral amino acids. Secreted heat-stable proteins were rich in Gix and proline. The possibilities that the accumulation of the heat-stable polypeptides during grain development is controlled by ABA and that the function of these polypeptides is related to their abundance and extraordinary heat stability are considered.This publication has 20 references indexed in Scilit:
- Why is one Bacillus alpha-amylase more resistant against irreversible thermoinactivation than another?Journal of Biological Chemistry, 1988
- Accumulation kinetics of cotton late embryogenesis-abundant mRNAs and storage protein mRNAs: Coordinate regulation during embryogenesis and the role of abscisic acidDevelopmental Biology, 1987
- Mode of Action of Abscisic Acid in Barley Aleurone LayersPlant Physiology, 1986
- Cumulative effect of intragenic amino-acid replacements on the thermostability of a proteinNature, 1986
- Regulation by ABA of β-Conglycinin Expression in Cultured Developing Soybean CotyledonsPlant Physiology, 1985
- Nonspecific stabilization of stress-susceptible proteins by stress-resistant proteins: a model for the biological role of heat shock proteins.Proceedings of the National Academy of Sciences, 1982
- The Anatomy and Taxonomy of Protein StructurePublished by Elsevier ,1981
- Control of protein synthesis in barley aleurone layers by the plant hormones gibberellic acid and abscisic acidCell, 1980
- Electrostatic Effects in ProteinsScience, 1978
- Mechanisms of ThermophilyCRC Critical Reviews in Microbiology, 1978