Expression in Escherichia coli of the carboxy terminal domain of the BLAR sensory-transducer protein of Bacillus licheniformis as a water-soluble Mr 26 000 penicillin-binding protein
Open Access
- 15 June 1990
- journal article
- Published by Oxford University Press (OUP) in FEMS Microbiology Letters
- Vol. 70 (1) , 107-113
- https://doi.org/10.1016/0378-1097(90)90111-3
Abstract
A cloning vector has been constructed which allows production and export by Escherichia coli of the Met346-Arg601 carboxy terminal domain of the 601 aminoc acid BLAR sensory-transducer involved in ß-lactamase inducibility in BacillusLicheniformis. The polypeptide, referred to as BLAR-CTD, accumulates in the periplasm of E.coli in the form of a water-soluble, Mr 26 000 penicillin-binding protein. These data and homology searches suggest that BLAR has a membrane topology similar to that of other sensory-transducers involved in chemotaxis.Keywords
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