Growth, Terpenoid Production and Antibacterial Activity of anin vitroCulture of the LiverwortFossombronia pusilla

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Abstract
In vitro cultures of the liverwort, Fossombronia pusilla, have been initiated from spores. They grew well on Gamborg B5 medium supplemented with 2% saccharose and 12.5 µg/l vitamin B12. Undifferentiated cultures were obtained on Knop mineral solution with 4% glucose. The cultures produced the same constituents as collected material. Perrottetianal A and B, known substances from liverworts were isolated and characterized by spectroscopic methods as well as α-(-)-santonin. A hitherto unknown diterpene dialdehyde - 8-hydroxy-9-hydroperrottetianal A - could also be characterized. Furthermore, 7 terpenes were identified by GC/MS. A petrol ether extract and the isolated terpenes exerted antibacterial activity.