Crossed Immunoelectrophoresis of a Herpes Simplex Virus Type I-Specific Antigen: Immunological and Biochemical Characterization
- 1 November 1978
- journal article
- research article
- Published by Oxford University Press (OUP) in The Journal of Infectious Diseases
- Vol. 138 (5) , 639-643
- https://doi.org/10.1093/infdis/138.5.639
Abstract
Crossed immunoelectrophoresis of herpes simplex virus (HSV) antigens obtained from infected cells solubilized with the nonionic detergent Triton X-IOO has shown that antigen number 6 (Ag-6) is found in preparations from HSV type l-infected cells but not in HSV type 2 preparations. A monospecific antiserum was produced against Ag-6 by intracutaneous inoculation of the corresponding immunoprecipitate into rabbits. This antiserum neutralized HSV type I but not HSV type 2. The polypeptide composition of Ag-6 (molecular weight, 131,000 daltons) was determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the immunoprecipitate, using radiolabeled antigen. The multitude of information obtained demonstrates that crossed immunoelectrophoresis can be used preparatively, since specific immunoprecipitates represent a short cut for the attainment of immunoreactive viral protein entities useful for further immunological and biochemical analyses.Keywords
This publication has 0 references indexed in Scilit: