Lack of vacuolar proton ATPase association with the cytoskeleton in osteoclasts of osteosclerotic (oc/oc) mice

Open Access
Publisher Website
Google Scholar
Abstract
We examined the pathogenetic mechanism underlying the lack of bone resorption in osteosclerotic oc/oc mice. An immunoelectron microscopic analysis revealed that in the osteoclasts of these mice, no ruffled borders formed, and that vacuolar H+‐ATPase (V‐ATPase) was present throughout the cytoplasm but not on the apical membranes. The activity of V‐ATPase in oc/oc mice was similar to that in normal mice. In normal spleen cell‐derived osteoclast‐like cells (OCLs), immunoreactivity for V‐ATPase was detected in association with Triton X‐100‐insoluble cellular structure, but not in oc/oc spleen cell‐derived OCLs. Moreover, in renal proximal convoluted tubules of oc/oc mice, the basal striation did not form. These results suggest that osteosclerosis in oc/oc mice is possibly due to the dissociation of V‐ATPase and cytoskeleton in osteoclasts.