Standardized molecular typing of Candida albicans strains
- 1 December 1997
- Vol. 40 (9-10) , 369-372
- https://doi.org/10.1111/j.1439-0507.1997.tb00252.x
Abstract
A method is presented for the standardization of Candida albicans DNA fingerprinting, which is based on Southern hybridization of EcoRI-digested chromosomal DNA with the moderately repetitive DNA element CARE-2 and the subsequent rehybridization of the blots with a molecular size marker also included in each DNA sample. This method resulted in extremely precise alignment of all strain-specific CARE-2 hybridization patterns, even when analysed on different gels, and will enhance the accuracy of genetic relationship determinations in epidemiological studies including large numbers of strains.Keywords
This publication has 14 references indexed in Scilit:
- Epidemiology of nosocomial fungal infectionsClinical Microbiology Reviews, 1996
- Epidemiology of Fungal Infections: The Promise of Molecular TypingClinical Infectious Diseases, 1995
- Strain identification of Mycobacterium tuberculosis by DNA fingerprinting: recommendations for a standardized methodologyJournal of Clinical Microbiology, 1993
- Isolation, characterization, and sequencing of Candida albicans repetitive element 2Gene, 1992
- A Critical Review of Typing Methods for Candida albicans and Their ApplicationsCritical Reviews in Microbiology, 1991