Determination of eprinomectin in plasma by high-performance liquid chromatography with automated solid phase extraction and fluorescence detection
- 1 January 1998
- journal article
- research article
- Published by Royal Society of Chemistry (RSC) in The Analyst
- Vol. 123 (7) , 1525-1527
- https://doi.org/10.1039/a802093k
Abstract
A method is described for the determination of eprinomectin in plasma using high-performance liquid chromatography with fluorescence detection (excitation and emission wavelengths 355 and 465 nm, respectively). The fluorescent derivative was obtained by a condensation reaction with trifluoroacetic anhydride and N-methylimidazole. The method employs 1 ml plasma samples and gives linear calibration graphs (r = 0.999) over the concentration range studied (0.5–50 ng ml–1). Solid extraction using the benchmate procedure was used for sample preparation. This method permits the determination of eprinomectin at levels as low as 0.1 ng ml–1 and is suitable for the pharmacokinetic study of eprinomectin in animals.Keywords
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