Interferon Assay of High Sensitivity
- 1 December 1976
- journal article
- research article
- Published by Frontiers Media SA in Experimental Biology and Medicine
- Vol. 153 (3) , 511-513
- https://doi.org/10.3181/00379727-153-39580
Abstract
A method of aging cells is described which allows for an increase in sensitivity of the interferon assay. After reaching confluency the cell cultures are aged at 30.degree. C for 5 days, the growth medium is replenished, and the cells are incubated for an additional 24 h at 37.degree. C before applying interferon. Using cell cultures so treated ([human] diploid [fibroblast] cells HFS-1 or HR-203), and mouse [fibroblast] L-929 cells), the sensitivity of the interferon assay increase from 16- to 30-fold for human and up to 10-fold for mouse standard interferon.This publication has 1 reference indexed in Scilit: